Stoichiometry of the Topa Quinone Biogenesis Reaction in Copper Amine Oxidases
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چکیده
منابع مشابه
Tyrosine codon corresponds to topa quinone at the active site of copper amine oxidases.
The recently discovered organic cofactor of bovine serum amine oxidase, topa quinone, is an uncommon amino acid residue in the polypeptide backbone (Janes, S. M., Mu, D., Wemmer, D., Smith, A. J., Kaur, S., Maltby, D., Burlingame, A. L., and Klinman, J. P. (1990) Science 248, 981-987). The amine oxidase gene from the yeast Hansenula polymorpha has been cloned and sequenced (Bruinenberg, P. G., ...
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Copper amine oxidases are important enzymes, which contribute to the regulation of mono- and polyamine levels. Each monomer contains one Cu(II) ion and 2,4,5-trihydroxyphenylalanine (TPQ) as cofactors. They catalyze the oxidative deamination of primary amines to aldehydes with a ping-pong mechanism consisting of a transamination. The mechanism is followed by the transfer of two electrons to mol...
متن کاملCharacterization of topa quinone cofactor.
Electrochemical characterization of topa quinone (6-hydroxydopa quinone), the organic cofactor of copper-containing amine oxidases, has been performed with the aid of spectroscopy and ab initio energy minimization technique. Topa quinone exhibits a totally reversible cyclic voltammogram at a mercury electrode, which is ascribed to a two-step one-electron conversion between topa quinone and topa...
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Copper amine oxidases (CAOs) catalyse the oxidative de-amination of biogenic amines which are ubiquitous compounds essential for cell growth and proliferation. The enzymes are homodimers containing both topaquinone and a Cu(II) ions as cofactors at the active site of each subunit. After extraction and purification of chickpea (cicer arietinum) amine oxidase by chromatoghraphy, Km and Vmax of th...
متن کاملInhibition of chickpea seedling copper amine oxidases by tetraethylenepentamine
Copper amine oxidases are important enzymes, which contribute to the regulation of monoand polyamine levels. Each monomer contains one Cu(II) ion and 2,4,5-trihydroxyphenylalanine (TPQ) as cofactors. They catalyze the oxidative deamination of primary amines to aldehydes with a ping-pong mechanism consisting of a transamination. The mechanism is followed by the transfer of two electrons to molec...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 1999
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi9950802